Cells interact with each other and with the environment in a very precise and regulated manner. The interactions play a crucial role in many cellular processes and ensure the integrity of tissue structure.
Cell-to-cell interaction forms communication channels for transmitting signals or synchronizing the response, for example in muscle contraction. However, loss of the interactions can result in unfavorable conditions for cell growth and ultimately leads to cancer.
Q-Phase opens the gates for discovering how cells interact together to prevent cell death in unfavorable cultivation conditions.
Moreover, it provides visualization of contact inhibition disruption and its consequences for the development of uncontrolled cell proliferation, tumor formation, and metastasis.
Time-lapse QPI images: (A) Entosis, (B) Cell fusion with cannibalism, (C) Cannibalism without fusion, (D) Oncosis, (E) Reverse oncosis.
Magnification 10x (A-D), 20x (E) | Balvan J, et al.: Oxidative Stress Resistance in Metastatic Prostate Cancer: Renewal by Self-Eating. PLoS One, 2015
Prostate cancer cell culture PC3 treated with Plumbagin showing apoptosis, necrosis, and entosis at 10x magnification.
PC3 prostate cancer cell shows active ingestion of another cancer cell in a phenomenon called cannibalism
Movements of subcellular particles along cell processes
LiveCodim super-resolution fluorescence microscopy is a perfect tool for studying mechanisms of cell-cell interactions such as membrane receptors dynamics and clusterization, actin assembly, dynamics at contact zones, or activation of specific contact-induced signaling pathways.
COS-7 cells cytoskeleton | Actin cytoskeleton is visualized using Sir-Actin marker
Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)
Q-Phase is a patented type of holographic microscope with high detection sensitivity
Q-Phase represents an ideal solution for experts who desire precise automated segmentation of individual cells for subsequent data analysis. Q-Phase easily transforms cell features and dynamics into numerical data ideal for comparisons, correlations, and more detailed statistics.
From conventional to super-resolution microscopy
LiveCodim is a universal, super-resolution imaging platform, designed to interface with any standard fluorescence microscope. It is the solution for live imaging with high resolution and low phototoxicity.