New digital technologies could turn previous limitations in the field of microscopy into new possibilities. Advances in digital cameras pave the way for developing innovative microscopic techniques that can be used not only for visualization of cells but also for their tracking and analysis according to the specific features.
Q-Phase is a state-of-the-art live-cell imaging method that quantifies the cellular growth and dynamics in label-free conditions, thus without induced cell toxicity. The system constitutes a top choice imaging cytometry with the fully motorized acquisition of both multi-channel fluorescent and QPI.
Q-Phase image quality permits very accurate and fast cell segmentation enabling to sort of the cells into distinct cell populations based on morphology, cell mass, or dynamics. A complete analysis of your experiment can be processed using All output data can be engaged using Q-Phase customized software.
Q-Phase creates images that can be precisely segmented due to the almost zero values of the background providing an excellent backgroud-to-sample ratio.
Analysis of changes in cell mass and porphology is primarily performed by a Q-Phase cell analyzer. Different tpyes of cell death can be distinguished.
Real-time analysis of early apoptosis in prostate cancer cells (segmented with a red outline) shows a significant decrease in cell area while preserving cell dry mass
Real-time analysis of necrosis in prostate cancer cells (segmented with green outline) show stable or increase cell area with a sudden loss of cell dry mass (upper graph)
Feature blot comparing cell area and cell mass in apoptotic cell population versus necrotic cell population
Timeline average values of cell mass, area and DAPI in apoptotic and necrotic cells
48 hours of metastatic prostate cancer cells PC3 in culture
Vicar, T. et al.
Vicar, T. et al.
L. Štrbková, et al.
Quantitative Phase Imaging
Q-Phase is a patented type of holographic microscope with high detection sensitivity
Q-Phase represents an ideal solution for experts who desire precise automated segmentation of individual cells for subsequent data analysis. Q-Phase easily transforms cell features and dynamics into numerical data ideal for comparisons, correlations, and more detailed statistics.