Telight

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Cell cycle

The cell cycle is a dynamic process where cells tend to grow and duplicate their contents for the subsequent division. Inappropriate cell division or aberrations in cell cycle regulation can lead to significant pathological outcomes such as cancer. Cell cycle pattern can be dramatically impacted by stressful environmental conditions such as hypoxia and malnutrition. On the other hand, stem cells can display unusual features in their cell cycle pattern which is tightly connected to the differentiation state.

Cell growth is directly proportional to the increase in the cell dry mass during individual cell cycle phases. Using Q-Phase in cell cycle studies represents a great quantitative method for assessing the symmetric and asymmetric cell division, cell cycle arrest, subpopulations that escape the therapeutic cytotoxic effect, doubling time, and others, all within a label-free technology.

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Asymmetric cell division

The selected cell could divide into identical daughter cell in mass and morphology for self-renewal and a daughter progenitor cell which is smaller with a lower cell mass value. Changes in cell mass are shown in the time graph confirming the asymmetric division.  | Magnification 10x

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Symmetrical cell division

The selected cell divides symmetrically into two daughter cells with identical cell mass values. The generated daughter cells have a similar cell mass growth curve and similar morphology across the timeline as shown in Q-Phase cell analyzer software. | Magnification 10x

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Quiescent cell

Note the cell dry mass curve which is almost at a fixed value in all time points, despite the frequent changes in cell morphology and dynamics

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Cell death in FUCCI cell line. Merged QPI and fluorescence

Merged QPI and fluorescence.

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Cell division in the quantitative color map

Magnification 60x

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Rat sarcoma cell mitosis (LW13K2) in quantitative color map

Magnification 20x

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Cell death in FUCCI cell line

Left image for QPI, right image for fluorescence | Magnification 20x

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Cell death in FUCCI cell line

Left image for QPI, right image for fluorescence

Cell cycle and cell division deregulation are involved in a variety of diseases, especially in cancer, and represent the main study domains in cell biology. Dynamic visualization of the cell cycle through super-resolution LiveCodim imaging could bring new insights into the mechanism involved in this process. For example by visualizing microtubule functioning during mitosis.

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Z-stack visualization of COS-7cytokinesis | Cells are labeled for mitochondria outer membrane (TOM-20 immunolabelling, green) and microtubules (TMR- Red) | Z-stack were acquired every 1µm on 10µm range

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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3D visualization of mitotic spindles in COS-7 cells| Cells are labeled for microtubules (TMR- Red) | Color code used in the right panel represents the thickness of the sample with blue corresponding to 0 and yellow to 10µm (step: 1µm)

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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COS-7 cell during cell division (metaphase) | Visualization of actin cytoskeleton (SiR-Actin, purple), mitochondria outer membrane (TOM-20 immunolabelling, green), and microtubules (TMR- Red)

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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COS-7 cell division | 3D visualization of actin cytoskeleton (purple), mitochondria outer membrane (green), and microtubules (Red) | On the right panels, color code used represents the thickness of the sample with blue corresponding to 0 and yellow to 9.6µm (step: 1µm)

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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Maximum intensity projection visualization of COS-7 cell during cell division (metaphase) | Marker: actin cytoskeleton (purple), mitochondria outer membrane (green), and microtubules (Red) | Images show maximum intensity projection of 10 Z-stack done every 1µm on a range of 10µm

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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Microtubule array on HELA cells | Marker: Anti Beta-tubulin Immuno-labelling with Alexa-488 coupled secondary antibody

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

Products

Telight Q-Phase

Telight Q-Phase

Discover QPI!

Q-Phase is a patented type of holographic microscope with high detection sensitivity

Q-Phase represents an ideal solution for experts who desire precise automated segmentation of individual cells for subsequent data analysis. Q-Phase easily transforms cell features and dynamics into numerical data ideal for comparisons, correlations, and more detailed statistics.

Telight LiveCodim

Telight LiveCodim

From conventional to super-resolution microscopy

LiveCodim is a universal, super-resolution imaging platform, designed to interface with any standard fluorescence microscope. It is the solution for live imaging with high resolution and low phototoxicity.