Telight

Telight

Immunology

Dysregulation of immune cells function can result in many serious and fatal diseases that require to re-modulate the immune cells and restore their normal response. Monitoring of immune cells while interacting with self-cells, pathogens, and cancer promotes our understanding of immune response or its failure mechanisms.

Today, immunotherapy procedures such as engineered T cells represent a powerful new approach to targeting cancer and autoimmune disorders. However, there are concerns about safety and off-target toxicity, as well as the development of resistance.

Q-Phase opens avenues for studying immune cell mechanisms and reactions both in space and time which can target many research fields and examine immunotherapy in a label-free method.

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Interaction of CAR-T cells and macrophages with cancer cells

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Interaction of T-cell with metastatic cancer cell

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Interaction of CAR-T cells and macrophages with cancer cells

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Interaction of CAR -T cell with cancer cell ends in cancer cell death

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Interaction of CAR-T cells and macrophages with ovarian cancer cells

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Interaction of CAR-T cells and macrophages with ovarian cancer cells

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Interaction of CAR-T cells and macrophages with ovarian cancer cells

Magnification 10x

Superresolution microscopy is crucial for investigating cell-cell interaction and particularly, microbiological immunity (against bacteria and viruses).

Indeed, due to their small size (few nm to µm), studying cellular and molecular immune responses remains challenging in conventional optical microscopy, but this could be overcome using the LiveCodim module.

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Mitochondrial dynamics in MDCK cells | Marker: Mitotracker-Orange | Mitochondria movements are visualized with 1 image every 5 seconds

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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Microtubule array on HELA cells | Marker: Anti Beta-tubulin Immuno-labelling with Alexa-488 coupled secondary antibody

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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Focal adhesions in MDCK cells | Marker: Vinculin | Vinculin is a membrane-cytoskeletal protein i that is involved in linkage of integrin to the actin cytoskeleton

The MDCK cells expressing fluorescent Vinculin were a generous gift of the Nicolas Borghi team at the Jacques Monod institute: “Mechanotransduction: from cell surface to nucleus”

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COS-7 cells cytoskeleton |Actin cytoskeleton is visualized using Sir-Actin marker

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

Publications

Maarifi, G., Fernandez, J., Portilho, D.M., et al.

RanBP2 regulates the anti-retroviral activity of TRIM5α by SUMOylation at a predicted phosphorylated SUMOylation motif

Products

Telight Q-Phase

Telight Q-Phase

Discover QPI!

Q-Phase is a patented type of holographic microscope with high detection sensitivity

Q-Phase represents an ideal solution for experts who desire precise automated segmentation of individual cells for subsequent data analysis. Q-Phase easily transforms cell features and dynamics into numerical data ideal for comparisons, correlations, and more detailed statistics.

Telight LiveCodim

Telight LiveCodim

From conventional to super-resolution microscopy

LiveCodim is a universal, super-resolution imaging platform, designed to interface with any standard fluorescence microscope. It is the solution for live imaging with high resolution and low phototoxicity.