Observation and analysis of cells in a stromal extracellular matrix (ECM), for example, collagen and matrigel, are essential for a full understanding of cell behavior. ECM mimics the structure and composition of the in vivo environment and affects many cellular properties, particularly adhesion, migration, and differentiation.
Thanks to the coherence-gating effect, a unique feature enabled by Telight Q-Phase, the QPI image quality is not impaired by the presence of the disturbing matrix. Therefore, the Q-Phase allows precise cell observation and analysis in scattering media.
QPI of human breast adenocarcinoma cell line MCF-7 in collagen
Analysis of cell mass during cell migration in the extracellular matrix is possible with Q-Phase
Human fibrosarcoma cell movement inside fibril of collagen gel | The distribution of cell mass during migration was studied in mesenchymal and amoeboid types
QPI of human fibrosarcoma cells in collagen
Magnification 20x | Tolde, O. et al.: Quantitative phase imaging unravels new insight into dynamics of mesenchymal and amoeboid cancer cell invasion. Scientific Reports, 2018
The patented design of Q-Phase allows high-quality QPI in turbid media
APODOX nanomaterial induced cell death in LNCap cell line | Magnification 10x
The patented design of Q-Phase allows high-quality OPI even in a semi-transparent medium
Colorectal cancer cells in 0,15% phospholipid emulsion | Magnification 20x | Collakova, J. et al.: Coherence-controlled holographic microscopy enabled recognition of necrosis as the mechanism of cancer cells death after exposure to cytopathic turbid emulsion, Journal of Biomedical Optics, 2015
Quantitative phase imaging unravels new insight into dynamics of mesenchymal and amoeboid cancer cell invasion
Coherence-controlled holographic microscopy enabled recognition of necrosis as the mechanism of cancer cells death after exposure to cytopathic turbid emulsion
Coherence-controlled holographic microscopy in diffuse media
Quantitative phase imaging through scattering media by means of coherence-controlled holographic microscope
Quantitative Phase Imaging
Q-Phase is a patented holographic microscope with high detection sensitivity.
Q-Phase is an ideal solution for experts who desire precise automated segmentation of individual cells for subsequent data analysis. Q-Phase quickly transforms cell features and dynamics into numerical data suitable for comparisons, correlations, and more detailed statistics.