Telight

Telight

Stem cells

Studying stem cell types and their differentiation properties helps to understand how they mature into functionally specialized cells.

Stem cell applications hold great promise as potential replacement therapy in regenerative medicine and other health-related problems such as spinal injuries, type-1 diabetes, Alzheimer’s disease, burns, and cancer.

The two defining characteristics of a stem cell are perpetual self-renewal and the ability to differentiate into a specialized adult cell type.

Q-Phase technology provides high phase detection sensitivity, allowing for measuring the cell dry mass of divided cells in pg/µm2 and thus defines cells with the morphological asymmetric division.

In addition, the clarity of the produced images ensures a precise segmentation and identification of delicate cell processes for detailed morphological assessment during differentiation. Q-Phase fluorescent module works simultaneously with QPI to visualize the differentiation markers.

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Human embryonic stem cells division and proliferation in real-time

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Human embryonic stem cells division and proliferation in real-time

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Mouse embryonic fibroblasts in QPI mode

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Mouse embryonic fibroblasts in DIC mode

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Human embryonic stem cells division and proliferation

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Due to the fundamental function of stem cells in developmental biology and their potential use in regenerative medicine and rare genetic diseased treatment, the visualization of their differentiation gained high importance. LiveCodim super-resolution module enables the investigation of fundamental mechanisms driving stem cells on the molecular level.

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COS-7 cells cytoskeleton | Actin cytoskeleton is visualized using Sir-Actin marker

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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Focal adhesions in MDCK cells | Marker: Vinculin | Vinculin is a membrane-cytoskeletal protein involved in linkage of integrin to the actin cytoskeleton

The MDCK cells expressing fluorescent Vinculin were a generous gift of the Nicolas Borghi team at the Jacques Monod institute: “Mechanotransduction: from cell surface to nucleus”

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Mitochondrial dynamics in MDCK cells | Marker: Mitotracker-Orange | Mitochondria movements are visualized with 1 image every 5 seconds

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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Microtubule array on HELA cells | Marker: Anti Beta-tubulin Immuno-labelling with Alexa-488 coupled secondary antibody

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

Publications

Garita-Hernandez M., et al.

Optogenetic light sensors in retinal organoids

Products

Telight Q-Phase

Telight Q-Phase

Discover QPI!

Q-Phase is a patented type of holographic microscope with high detection sensitivity

Q-Phase represents an ideal solution for experts who desire precise automated segmentation of individual cells for subsequent data analysis. Q-Phase easily transforms cell features and dynamics into numerical data ideal for comparisons, correlations, and more detailed statistics.

Telight LiveCodim

Telight LiveCodim

From conventional to super-resolution microscopy

LiveCodim is a universal, super-resolution imaging platform, designed to interface with any standard fluorescence microscope. It is the solution for live imaging with high resolution and low phototoxicity.