Cell migration is a fundamental dynamic process occurring in physiological (immune cells) and pathological conditions (cancer cells).
The mechanisms of cell migration largely depend on cell type and the guiding cellular environment where cells adapt their microenvironment to migrate efficiently. Innovative microscopic technologies allowing for live-cell imaging open a new horizon for studying cell migration in 2D and 3D environments.
Cell migration mechanisms largely depend on cell type and the guiding cellular environment. Thus, cells acquire specific migratory phenotypes to migrate efficiently through tissues and adapt to their microenvironment. Motile cells are polarized, and the actin cytoskeleton is a key modulator of cell polarity and pseudopods whose stabilization determines the directionality of the movement.
Q-Phase defines the pattern of cell polarity through assessment of the morphological profile of migratory cells together with the measurement of their core and peripheral dry mass distribution.
QPI of human breast adenocarcinoma cell line MCF-7 in collagen
Analysis of cell mass during cell migration in the extracellular matrix is possible now with Q-Phase
Human fibrosarcoma cell movement inside fibrils of collagen gel | The distribution of cell mass during migration was studied in mesenchymal and amoeboid types
Migration of human chondrogenic cells
Migration of human chondrogenic cells
QPI time-lapse of untreated PC3 cells
Marked cell shows a transition to fast amoeboid migration phenotype which involves a myosin-II-dependent mechanical instability of the cell cortex | Magnification 10x
Phase image and a quantitative colormap for the fast amoeboid migration phenotype of PC3 cell
Quantitative values are automatically calculated in pg/µm²
Deregulation of cell migration is involved in cancer progression and metastatic development. Thus it constitutes a major research topic of interest for decades already.
In this context, the LiveCodim can represent the perfect choice to dynamically image the actin cytoskeleton and visualize the specific structures involved in cell migration and invasion in superresolution, such as actin stress fibers, filopodia, lamellipodia, endosomes, etc.
Focal adhesions in MDCK cells | Marker: Vinculin | Vinculin is a membrane-cytoskeletal protein that is involved in linkage of integrin to the actin cytoskeleton
The MDCK cells expressing fluorescent Vinculin were a generous gift of the Nicolas Borghi team at the Jacques Monod institute: “Mechanotransduction: from cell surface to nucleus”
COS-7 cells cytoskeleton | Actin cytoskeleton is visualized using Sir-Actin marker
Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)
Quantitative phase imaging unravels new insight into dynamics of mesenchymal and amoeboid cancer cell invasion
Fojtů, M. et al.
Silicane Derivative Increases Doxorubicin Efficacy in an Ovarian Carcinoma Mouse Model: Fighting Drug Resistance
Cisplatin enhances cell stiffness and decreases invasiveness rate in prostate cancer cells by actin accumulation
Quantitative Phase Imaging of Spreading Fibroblasts Identifies the Role of Focal Adhesion Kinase in the Stabilization of the Cell Rear
Quantitative Phase Imaging
Q-Phase is a patented holographic microscope with high detection sensitivity.
Q-Phase is an ideal solution for experts who desire precise automated segmentation of individual cells for subsequent data analysis. Q-Phase quickly transforms cell features and dynamics into numerical data suitable for comparisons, correlations, and more detailed statistics.
From conventional to super-resolution microscopy
LiveCodim is a universal, super-resolution imaging platform, designed to interface with any standard fluorescence microscope. It is the solution for live imaging with high resolution and low phototoxicity.