Telight

Telight

Migration studies

Cell migration is a fundamental dynamic process occurring in many physiological (immune cells) and pathological conditions (cancer cells).

The mechanisms of cell migration largely depend on cell type and the guiding cellular environment where cells adapt their microenvironment to migrate efficiently. Innovative microscopic technologies allowing for live-cell imaging opened a new horizon for studying cell migration in 2D and 3D environments.

Cell migration mechanisms largely depend on cell type and the guiding cellular environment. Thus, cells acquire specific migratory modes and phenotype to migrate efficiently through tissues and adapt to their microenvironment. Motile cells are polarized, and the actin cytoskeleton is a key modulator of cell polarity and pseudopods whose stabilization determines the directionality of the movement.

Q-Phase defines the pattern of cell polarity through assessment of the morphological profile of migratory cells together with the measurement of their core and peripheral dry mass distribution.

Preview image

QPI of human breast adenocarcinoma cell line MCF-7 in collagen

Magnification 20x

Preview video image

Analysis of cell mass during cell migration in the extracellular matrix is possible now with Q-Phase

Human fibrosarcoma cell movement inside fibrils of collagen gel | The distribution of cell mass during migration was studied in mesenchymal and amoeboid types

Preview video image

Migration of human chondrogenic cells

Magnification 20x

Preview video image

Migration of human chondrogenic cells

Magnification 20

Preview video image

QPI time-lapse of untreated PC3 cells

Marked cell shows a transition to fast amoeboid migration phenotype which involves a myosin-II-dependent mechanical instability of the cell cortex | Magnification 10x

Preview image

Phase image and a quantitative colormap for the fast amoeboid migration phenotype of PC3 cell

Quantitative values are automatically calculated in pg/µm²

Deregulation of cell migration processes is involved in cancer progression and metastatic development and thus constitutes a major research topic of interest for decades already.

In this context, the LiveCodim can represent the perfect choice to dynamically image the actin cytoskeleton and visualize the specific structures involved in cell migration and invasion in superresolution, such as actin stress fibers, filopodia, lamellipodia, endosomes, etc.

Preview image

Focal adhesions in MDCK cells | Marker: Vinculin | Vinculin is a membrane-cytoskeletal protein i that is involved in linkage of integrin to the actin cytoskeleton

The MDCK cells expressing fluorescent Vinculin were a generous gift of the Nicolas Borghi team at the Jacques Monod institute: “Mechanotransduction: from cell surface to nucleus”

Preview image

COS-7 cells cytoskeleton | Actin cytoskeleton is visualized using Sir-Actin marker

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

Products

Telight Q-Phase

Telight Q-Phase

Discover QPI!

Q-Phase is a patented type of holographic microscope with high detection sensitivity

Q-Phase represents an ideal solution for experts who desire precise automated segmentation of individual cells for subsequent data analysis. Q-Phase easily transforms cell features and dynamics into numerical data ideal for comparisons, correlations, and more detailed statistics.

Telight LiveCodim

Telight LiveCodim

From conventional to super-resolution microscopy

LiveCodim is a universal, super-resolution imaging platform, designed to interface with any standard fluorescence microscope. It is the solution for live imaging with high resolution and low phototoxicity.