Super-resolution microscopy

With the development of new techniques, fluorescence optical microscopy can now help biologists decipher the dynamics of biological processes at the scale of a few tenths of a nanometer.

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LiveCodim, is a super-resolution solution for every lab. Book a demo with us and see how easy it is to upgrade your confocal microscope to a super-resolution microscope.

LiveCodim uses a unique patented laser beam shaper. Our technology perfectly fits the needs of biologists in super-resolution and is adapted for living samples, to visualize cellular and molecular dynamics in high resolution.

COS-7 cells cytoskeleton |Actin cytoskeleton is visualized using Sir-Actin marker

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

Microtubule array on HELA cells | Marker: Anti Beta-tubulin Immuno-labelling with Alexa-488 coupled secondary antibody

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

Mitochondrial dynamics in MDCK cells | Marker: Mitotracker-Orange | Mitochondria movements are visualized with 1 image every 5 seconds

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

Focal adhesions in MDCK cells | Marker: Vinculin | Vinculin is a membrane-cytoskeletal protein involved in linkage of integrin to the actin cytoskeleton

The MDCK cells expressing fluorescent Vinculin were a generous gift of the Nicolas Borghi team at the Jacques Monod institute: “Mechanotransduction: from cell surface to nucleus”

COS-7 cells cytoskeleton | Actin cytoskeleton is visualized using Sir-Actin marker

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

Z-stack visualization of COS-7cytokinesis | Cells are labeled for mitochondria outer membrane (TOM-20 immunolabelling, green) and microtubules (TMR- Red). Z-stack were acquired every 1µm on 10µm range

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

3D visualization of COS-7 cytokinesis | Cells are labeled for mitochondria outer membrane (TOM-20 immunolabelling, green) and microtubules (TMR- Red). Color code used in the lower panel represents the thickness of the sample with blue corresponding to 0 and yellow to 10µm (step: 1µm)

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

Comparison of widefield and super-resolution visualization of fixed COS-7 cells labeled with 4 fluorescent markers | Nucleus: DAPI, Mitochondria: Anti-Tom20 with Alexa Fluor 488, Microtubules: Anti-Tubulin with TMR, Actin: SiR

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

Confocal (made with LiveCodim) visualization of COS-7 cells labeled with 4 fluorescent markers | Nucleus: DAPI, Mitochondria: Anti-Tom20 with Alexa Fluor 488, Microtubules: Anti-Tubulin with TMR, Actin: SiR

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

Comparison of widefield, confocal, and super-resolution of microtubule visualization in COS-7 cells | Marker: Anti-beta tubulin antibody and TMR coupled secondary antibody

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France

3D visualization of mitotic spindles in COS-7 cells| Cells are labeled for microtubules (TMR- Red). Color code used in the right panel represents the thickness of the sample with blue corresponding to 0 and yellow to 10µm (step: 1µm)

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

COS-7 cell during cell division (metaphase) | Visualization of actin cytoskeleton (SiR-Actin, purple), mitochondria outer membrane (TOM-20 immunolabelling, green), and microtubules (TMR- Red)

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

Maximum Intensity projection visualization of COS-7 cell during cell division (metaphase) | Marker: actin cytoskeleton (purple), mitochondria outer membrane (green), and microtubules (Red). Images show maximum intensity projection of 10 Z-stack done every 1µm on a range of 10µm

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

COS-7 cell division | 3D visualization of actin cytoskeleton (purple), mitochondria outer membrane (green), and microtubules (Red). On the right panels, color code used represents the thickness of the sample with blue corresponding to 0 and yellow to 9.6µm (step: 1µm)

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

Widefield, confocal, and super-resolution visualization of MDCK cells expressing fluorescent Vinculin (green) and labeled with mitotracker-Orange (red)

The MDCK cells expressing fluorescent Vinculin were a generous gift of the Nicolas Borghi team at the Jacques Monod institute: “Mechanotransduction: from the cell surface to nucleus”

2 color dynamic of mitochondria and Vinculin in MDCK cell | Cell expressing fluorescent Vinculin were labeled using mitotracker-Orange and images every 2min for 10 min.

The MDCK cells expressing fluorescent Vinculin were a generous gift of the Nicolas Borghi team at the Jacques Monod institute: “Mechanotransduction: from the cell surface to nucleus”

Dynamic of mitochondria in MDCK cells Cells are labeled with mitotracker-orange (1 image acquisition every 1 min for 30 min)

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

Publications

J. Vargas, et al.

The Wnt/Ca2+ pathway is involved in interneuronal communication mediated by tunneling nanotubes

EMBO Journal, 2019 Download PDF

Maarifi, G., Fernandez, J., Portilho, D.M., et al.

RanBP2 regulates the anti-retroviral activity of TRIM5α by SUMOylation at a predicted phosphorylated SUMOylation motif

Communications Biology, 2018 Download PDF

Garita-Hernandez M., et al.

Optogenetic light sensors in retinal organoids

Frontiers in Neuroscience, 2018

Getz A.M., Xu F., Visser F., et al.

Tumor suppressor menin is required for subunit-specific nAChR Alpha5 transcription and nAChR-dependent presynaptic facilitation in cultured mouse hippocampal neurons

Scientific Reports (Nature), 2017 Download PDF

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From conventional to super-resolution microscopy

LiveCodim is a universal, super-resolution imaging platform designed to interface with any standard fluorescence microscope. It is the solution for live-cell imaging with high resolution and low phototoxicity.