Telight

Telight

Virology

The virus is a submicroscopic structure containing genetic material that can not replicate without the machinery and metabolism of host cells. A viral infection is usually associated with morphological and pathological cellular changes that are necessary for efficient viral replication and may end in host cell death.

Time-lapse microscopy systems are essential for understanding the key factors influencing the viral replication steps and their effect on host cells.

Viral cytopathic effects on cell morphology are commonly seen as rounding of the infected cell, fusion with adjacent cells (syncytia), and the appearance of nuclear or cytoplasmic inclusion bodies. The virus can also alter the cellular activities of the host cell, such as their cell cycle.

Q-Phase can monitor the influence of the virus on cells, mode of cell death, and immune response, all of that in real-time. The ability to use both QPI and Fluorescence in a single experiment enables to probe the viral particles and detect their development inside cells in real-time.

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Human embryonic kidney cells transfected with GFP Vesicular Stomatitis Virus coat protein

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EK293 cell population transfected with GFP Vesicular Stomatitis Virus coat protein

DIC images automatically reconstructed from phase images and merged with Fluorescence to visualize the viral infected cells

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HEK293 cell population transfected with GFP Vesicular Stomatitis Virus coat protein

QPI images merged with Fluorescence to visualize the viral infected cells

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HEK293 cell population transfected with GFP Vesicular Stomatitis Virus coat protein

QPI images are merged with Fluorescence to visualize the viral infected cells

Due to their small size ranging between 20-200nm, imaging of viruses in optical microscopy has been challenging for decades.

In this context, super-resolution techniques including LiveCodim will be of interest, for example for visualizing different steps of cellular infection such as membrane interaction, transfer of genetic material, viral replication, etc.

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COS-7 cells cytoskeleton |Actin cytoskeleton is visualized using Sir-Actin marker

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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Microtubule array on HELA cells | Marker: Anti Beta-tubulin Immuno-labelling with Alexa-488 coupled secondary antibody

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

Publications

L. Eyer, et al.

Broad-Spectrum Antiviral Activity of 3’-Deoxy-3’-Fluoroadenosine against Emerging Flaviviruses

Maarifi, G., Fernandez, J., Portilho, D.M., et al.

RanBP2 regulates the anti-retroviral activity of TRIM5α by SUMOylation at a predicted phosphorylated SUMOylation motif

Portilho D.M., Persson R., Arhel N.

Role of non-motile microtubule-associated proteins in virus trafficking

Products

Telight Q-Phase

Telight Q-Phase

Discover QPI!

Q-Phase is a patented type of holographic microscope with high detection sensitivity

Q-Phase represents an ideal solution for experts who desire precise automated segmentation of individual cells for subsequent data analysis. Q-Phase easily transforms cell features and dynamics into numerical data ideal for comparisons, correlations, and more detailed statistics.

Telight LiveCodim

Telight LiveCodim

From conventional to super-resolution microscopy

LiveCodim is a universal, super-resolution imaging platform, designed to interface with any standard fluorescence microscope. It is the solution for live imaging with high resolution and low phototoxicity.