Refractive index
The refractive index of a tissue is a measure of light speed reduction in a given tissue. In other words, the higher the refractive index the slower the light travels.
Cell refractive index is a key biophysical parameter that represents the intracellular mass density and provides important insight for research fields like cell biology, hematology, and pathology. Also, studying the refractive indices of biosensors can bring discoveries and reveal novel applications.
Refractive index (RI) can be calculated from the phase shift values measured by Q-Phase using two methods. When the thickness of the sample is known, RI values can be calculated directly from the detected phase shift. And if the thickness of a sample is not known, the phase decoupling method can be used.
In this approach, the RI distribution is estimated from the phase shift measured on two occasions when the sample is being serially embedded in two different media with known RI.
Publications
Spaeker, O. et al.
Gradients of orientation, composition and hydration of proteins for efficient light collection by the cornea of the horseshoe crab
Vicar, T. et al.
Cancer Cells Viscoelasticity Measurement by Quantitative Phase and Flow Stress Induction
Products
Telight Q-Phase
Quantitative Phase Imaging
Q-Phase is a patented holographic microscope with high detection sensitivity.
Q-Phase is an ideal solution for experts who desire precise automated segmentation of individual cells for subsequent data analysis. Q-Phase quickly transforms cell features and dynamics into numerical data suitable for comparisons, correlations, and more detailed statistics.