Telight

Telight

Super-resolution

With the development of new techniques, fluorescence optical microscopy can now help biologists to decipher the dynamics of biological processes at the scale of a few tenths of a nanometer.

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LiveCodim, super-resolution solution for every lab

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Super-resolution in light microscopy

EMBO Practical Course

September 20 – 22

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LiveCodim uses a unique patented laser beam shaper. Our technology perfectly fits the needs of biologists in super resolution and is adapted for living samples, to visualize cellular and molecular dynamics in high resolution.

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COS-7 cells cytoskeleton |Actin cytoskeleton is visualized using Sir-Actin marker

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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Microtubule array on HELA cells | Marker: Anti Beta-tubulin Immuno-labelling with Alexa-488 coupled secondary antibody

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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Mitochondrial dynamics in MDCK cells | Marker: Mitotracker-Orange | Mitochondria movements are visualized with 1 image every 5 seconds

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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Focal adhesions in MDCK cells | Marker: Vinculin | Vinculin is a membrane-cytoskeletal protein involved in linkage of integrin to the actin cytoskeleton

The MDCK cells expressing fluorescent Vinculin were a generous gift of the Nicolas Borghi team at the Jacques Monod institute: “Mechanotransduction: from cell surface to nucleus”

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COS-7 cells cytoskeleton | Actin cytoskeleton is visualized using Sir-Actin marker

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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Z-stack visualization of COS-7cytokinesis | Cells are labeled for mitochondria outer membrane (TOM-20 immunolabelling, green) and microtubules (TMR- Red). Z-stack were acquired every 1µm on 10µm range

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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3D visualization of COS-7 cytokinesis | Cells are labeled for mitochondria outer membrane (TOM-20 immunolabelling, green) and microtubules (TMR- Red). Color code used in the lower panel represents the thickness of the sample with blue corresponding to 0 and yellow to 10µm (step: 1µm)

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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Comparison of widefield and super-resolution visualization of fixed COS-7 cells labeled with 4 fluorescent markers | Nucleus: DAPI, Mitochondria: Anti-Tom20 with Alexa Fluor 488, Microtubules: Anti-Tubulin with TMR, Actin: SiR

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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Confocal (made with LiveCodim) visualization of COS-7 cells labeled with 4 fluorescent markers | Nucleus: DAPI, Mitochondria: Anti-Tom20 with Alexa Fluor 488, Microtubules: Anti-Tubulin with TMR, Actin: SiR

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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Comparison of widefield, confocal, and super-resolution of microtubule visualization in COS-7 cells | Marker: Anti-beta tubulin antibody and TMR coupled secondary antibody

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France

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3D visualization of mitotic spindles in COS-7 cells| Cells are labeled for microtubules (TMR- Red). Color code used in the right panel represents the thickness of the sample with blue corresponding to 0 and yellow to 10µm (step: 1µm)

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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COS-7 cell during cell division (metaphase) | Visualization of actin cytoskeleton (SiR-Actin, purple), mitochondria outer membrane (TOM-20 immunolabelling, green), and microtubules (TMR- Red)

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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Maximum Intensity projection visualization of COS-7 cell during cell division (metaphase) | Marker: actin cytoskeleton (purple), mitochondria outer membrane (green), and microtubules (Red). Images show maximum intensity projection of 10 Z-stack done every 1µm on a range of 10µm

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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COS-7 cell division | 3D visualization of actin cytoskeleton (purple), mitochondria outer membrane (green), and microtubules (Red). On the right panels, color code used represents the thickness of the sample with blue corresponding to 0 and yellow to 9.6µm (step: 1µm)

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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Widefield, confocal, and super-resolution visualization of MDCK cells expressing fluorescent Vinculin (green) and labeled with mitotracker-Orange (red)

The MDCK cells expressing fluorescent Vinculin were a generous gift of the Nicolas Borghi team at the Jacques Monod institute: “Mechanotransduction: from the cell surface to nucleus”

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2 color dynamic of mitochondria and Vinculin in MDCK cell | Cell expressing fluorescent Vinculin were labeled using mitotracker-Orange and images every 2min for 10 min.

The MDCK cells expressing fluorescent Vinculin were a generous gift of the Nicolas Borghi team at the Jacques Monod institute: “Mechanotransduction: from the cell surface to nucleus”

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Dynamic of mitochondria in MDCK cells Cells are labeled with mitotracker-orange (1 image acquisition every 1 min for 30 min)

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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Telight LiveCodim

Telight LiveCodim

From conventional to super-resolution microscopy

LiveCodim is a universal, super-resolution imaging platform, designed to interface with any standard fluorescence microscope. It is the solution for live imaging with high resolution and low phototoxicity.