Telight

Telight

Super-resolution

With the development of new techniques, fluorescence optical microscopy can now help biologists to decipher the dynamics of biological processes at the scale of a few tenths of a nanometer.

LiveCodim – online official launch session

May 26, 2021 | 2 p.m.

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LiveCodim uses a unique patented laser beam shaper. Our technology perfectly fits the needs of biologists in superresolution and is adapted for living samples, to visualize cellular and molecular dynamics in high-resolution.

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COS-7 cells cytoskeleton |Actin cytoskeleton is visualized using Sir-Actin marker

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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Microtubule array on HELA cells | Marker: Anti Beta-tubulin Immuno-labelling with Alexa-488 coupled secondary antibody

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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Mitochondrial dynamics in MDCK cells | Marker: Mitotracker-Orange | Mitochondria movements are visualized with 1 image every 5 seconds

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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Focal adhesions in MDCK cells | Marker: Vinculin | Vinculin is a membrane-cytoskeletal protein involved in linkage of integrin to the actin cytoskeleton

The MDCK cells expressing fluorescent Vinculin were a generous gift of the Nicolas Borghi team at the Jacques Monod institute: “Mechanotransduction: from cell surface to nucleus”

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COS-7 cells cytoskeleton | Actin cytoskeleton is visualized using Sir-Actin marker

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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Z-stack visualization of COS-7cytokinesis | Cells are labeled for mitochondria outer membrane (TOM-20 immunolabelling, green) and microtubules (TMR- Red). Z-stack were acquired every 1µm on 10µm range

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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3D visualization of COS-7 cytokinesis | Cells are labeled for mitochondria outer membrane (TOM-20 immunolabelling, green) and microtubules (TMR- Red). Color code used in the lower panel represents the thickness of the sample with blue corresponding to 0 and yellow to 10µm (step: 1µm)

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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Comparison of widefield and super-resolution visualization of fixed COS-7 cells labeled with 4 fluorescent markers | Nucleus: DAPI, Mitochondria: Anti-Tom20 with Alexa Fluor 488, Microtubules: Anti-Tubulin with TMR, Actin: SiR

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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Confocal (made with LiveCodim) visualization of COS-7 cells labeled with 4 fluorescent markers | Nucleus: DAPI, Mitochondria: Anti-Tom20 with Alexa Fluor 488, Microtubules: Anti-Tubulin with TMR, Actin: SiR

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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Comparison of widefield, confocal, and super-resolution of microtubule visualization in COS-7 cells | Marker: Anti-beta tubulin antibody and TMR coupled secondary antibody

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France

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3D visualization of mitotic spindles in COS-7 cells| Cells are labeled for microtubules (TMR- Red). Color code used in the right panel represents the thickness of the sample with blue corresponding to 0 and yellow to 10µm (step: 1µm)

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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COS-7 cell during cell division (metaphase) | Visualization of actin cytoskeleton (SiR-Actin, purple), mitochondria outer membrane (TOM-20 immunolabelling, green), and microtubules (TMR- Red)

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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Maximum Intensity projection visualization of COS-7 cell during cell division (metaphase) | Marker: actin cytoskeleton (purple), mitochondria outer membrane (green), and microtubules (Red). Images show maximum intensity projection of 10 Z-stack done every 1µm on a range of 10µm

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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COS-7 cell division | 3D visualization of actin cytoskeleton (purple), mitochondria outer membrane (green), and microtubules (Red). On the right panels, color code used represents the thickness of the sample with blue corresponding to 0 and yellow to 9.6µm (step: 1µm)

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

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Widefield, confocal, and super-resolution visualization of MDCK cells expressing fluorescent Vinculin (green) and labeled with mitotracker-Orange (red)

The MDCK cells expressing fluorescent Vinculin were a generous gift of the Nicolas Borghi team at the Jacques Monod institute: “Mechanotransduction: from the cell surface to nucleus”

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2 color dynamic of mitochondria and Vinculin in MDCK cell | Cell expressing fluorescent Vinculin were labeled using mitotracker-Orange and images every 2min for 10 min.

The MDCK cells expressing fluorescent Vinculin were a generous gift of the Nicolas Borghi team at the Jacques Monod institute: “Mechanotransduction: from the cell surface to nucleus”

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Dynamic of mitochondria in MDCK cells Cells are labeled with mitotracker-orange (1 image acquisition every 1 min for 30 min)

Institut Jacques Monod, Imagoseine imaging facility (CNRS UMR7592 and Université de Paris, France)

Products

Telight LiveCodim

Telight LiveCodim

From conventional to super-resolution microscopy

LiveCodim is a universal, super-resolution imaging platform, designed to interface with any standard fluorescence microscope. It is the solution for live imaging with high resolution and low phototoxicity.